Assessment of minimum growth conditions for the in vitro conservation of Vernonia condensata

Authors

DOI:

https://doi.org/10.46357/bcnaturais.v18i1.792

Keywords:

Medicinal plants, Micropropagation, Slow growth

Abstract

This work aims to establish minimum growth conditions for the development of an in vitro conservation protocol for Vernonia condensata Baker, to be used in research and to be inserted in public health service in the Recôncavo da Bahia. Nodal segments of approximately 1.5 cm in size were used, from V. condensata cultivated in vitro beforehand. These explants were inoculated into test tubes containing MS culture medium (MS, MS/2 and MS/4), supplemented with 15 g L-1 and 30 g L-1 of sucrose, kept in a growth room (25 ± 1 ºC) and in a BOD (20 ± 1 ºC) for 120 days. Evaluations were carried out at intervals of 30 days of cultivation, using the variables: plant height, number of green leaves, number of senescent leaves and number of roots. The MS with the addition of 15 g L-1 of sucrose provided the lowest in vitro growth of V. condensata plants. For the maintenance of V. condensata plants for a period of 120 days with lower height and satisfactory number of green leaves, an MS culture medium should be considered with 15 g L-1 of sucrose associated with a culture environment at a temperature of 25 ºC.

Author Biographies

  • Rafaela Fonseca Lopes, Centro Universitário Maria Milza. Governador Mangabeira, Bahia, Brasil
    Graduated in Nursing from Faculty Maria Milza (2010). Master in the Regional Development
    and Environment Program. Specialist in Public Health, Permanent Education and Obstetrics.
    Doula. She is currently a professor at Faculty Maria Milza, Nurse at the Nursing Outpatient
    Clinic for Women and Coordinator of Primary Care at the Municipal Health Secretariat of
    Sapeaçu-BA. She has experience in the field of Nursing, with an emphasis on Public Health,
    Continuing Education in Health and Women's Health.
  • Karolina Silva Leite Santana, Centro Universitário Maria Milza. Governador Mangabeira, Bahia, Brasil
    Undergraduate in Biomedicine, Faculty Maria Milza, scholarship holder of the Scientific
    Initiation Program (PROINC/FAPESB) in the period 2019-2020, with research focused on plant
    tissue culture. Member of the Research Group on Plant Biotechnology Applied to Health and
    Sustainability at Faculdade Maria Milza (FAMAM)
  • Vânia de Jesus dos Santos, Centro Universitário Maria Milza. Governador Mangabeira, Bahia, Brasil
    Bachelor's Degree in Biomedicine, Faculty Maria Milza, scholarship holder Graduated in
    Agronomic Engineering at the Federal University of Bahia (2006). Degree in Biology from
    the Faculty of Social Sciences (2016). Specialist in Research Methodology from the Faculty
    Maria Milza (2015). Master in Agricultural Sciences from the Federal University of
    Recôncavo da Bahia (2009). Doctor by the Postgraduate Program in Agricultural Sciences at
    UFRB (2012). I teach at Faculty Maria Milza in the courses of Pharmacy, Biomedicine,
    Nutrition, Physiotherapy and Administration, I also act as coordinator of the FAMAM
    laboratories, and I advise on the Master's Programs in Regional Development and
    Environment and in Biotechnology. Area of ​​expertise: Molecular Biology, Genetics and
    Molecular Markers applied to pineapple, banana, cassava and transgenic papaya crops,
    Plant Physiology, Post ? harvest (physical - chemical analysis), Medicinal Plants
    (Ethnobotany, Quality Control and In vitro Establishment), Scientific Methodology and
    Phytopathology. The Scientific Initiation Program (PROINC/FAPESB) in the period 2019-2020,
    with research focused on culture of plant tissues. Member of the Research Group on Plant
    Biotechnology Applied to Health and Sustainability at Faculdade Maria Milza (FAMAM)

     

  • Eva Maria Rodrigues Costa, Centro Universitário Maria Milza. Governador Mangabeira, Bahia, Brasil

     

    Graduated in Agronomic Engineering from the Federal University of Piauí (2007), Master in
    Agronomy (Genetic Plant Improvement) from the Federal Rural University of Pernambuco (2010)
    and Ph.D. in Genetics and Plant Improvement from the Federal University of Lavras (2013).

     

  • Mariane de Jesus da Silva de Carvalho, Centro Universitário Maria Milza. Governador Mangabeira, Bahia, Brasil
    Graduated in Agricultural Engineering from the Federal University of Recôncavo da Bahia
    (2010), Master in Plant Genetic Resources (2013) and Ph.D. in Agricultural Sciences from
    the same educational institution (2016). She is currently Permanent Professor of the
    Professional Master's Program in Regional Development and Environment and the Professional
    Master's Program in Biotechnology at Faculty Maria Milza - FAMAM and, in addition,
    Professor of Biostatistics (Graduate Courses in Nursing and Pharmacy) and Statistics
    ( Undergraduate courses in Accounting and Administration) from the same educational
    institution. He works in the areas of Plant Genetic Improvement, Plant Genetic Resources,
    Statistics and Agricultural Experimentation, with an emphasis on experiment planning,
    statistical data analysis and tissue culture of banana, bromeliads, citrus, cassava,
    orchids and medicinal plants.  
  • Weliton Antônio Bastos de Almeida de Almeida, Centro Universitário Maria Milza. Governador Mangabeira, Bahia, Brasil
    Graduated in Agronomy from the SCHOOL OF AGRONOMY OF THE FEDERAL UNIVERSITY OF BAHIA (1985,
    Master in Agricultural Sciences from the Federal University of Bahia (1994) and Doctorate
    in Plant Science from the University of São Paulo (2002). He is currently Director of Honor
    at Faculdade Maria Milza and Retired Adjunct Professor at the Federal University of
    Recôncavo da Bahia. Has experience in Agronomy, focusing on Plant Anatomy and Organography,
    acting on the following subjects: biotechnology, in vitro cultivation, micropropagation,
    tissue culture, plant anatomy and medicinal plants. Collaborator of the Graduate Programs
    at Faculdade Maria Milza: 1 - Master's Degree in Regional Development and Environment;
    2 - Master's Degree in Biotechnology.

     

Published

04/28/2023

How to Cite

Lopes, R. F., Santana, K. S. L., dos Santos, V. de J., Costa, E. M. R., de Carvalho, M. de J. da S., & de Almeida, W. A. B. de A. (2023). Assessment of minimum growth conditions for the in vitro conservation of Vernonia condensata. Boletim Do Museu Paraense Emílio Goeldi - Ciências Naturais, 18(1), 1-13. https://doi.org/10.46357/bcnaturais.v18i1.792